Composite surface for blocking bacterial adsorption on protein biochips
نویسندگان
چکیده
منابع مشابه
Composite surface for blocking bacterial adsorption on protein biochips.
The design and fabrication of protein biochips requires characterization of blocking agents that minimize nonspecific binding of proteins or organisms. Nonspecific adsorption of Escherichia coli, Listeria innocua, and Listeria monocytogenes is prevented by bovine serum albumin (BSA) or biotinylated BSA adsorbed on SiO(2) surfaces of a biochip that had been modified with a C(18) coating. Biotiny...
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Lysozyme placed on the SiO2 surfaces that have previously been derivatized with C18 coating will capture both Escherichia coli and Listeria monocytogenes cells from PBS buffer at pH 7.2. This phenomenon is of significance for the design and fabrication of protein biochips that are designed to capture bacteria from buffer or water so that these can be further interrogated with respect to possibl...
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ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 2003
ISSN: 0006-3592,1097-0290
DOI: 10.1002/bit.10507